In vitro Anthelmintic Activity of Croton tiglium Seeds Extract on Haemonchus contortus

Background: Haemonchus contortus is a blood sucking parasite whose habitat the abomasum of ruminants, which poses significant threat to health and production sheep goats in warm temperate tropical regions. H. leads anemia, hypoproteinemia, reduced exercise tolerance edema subcutaneous tissue that can result death animals. In acute infection, animals suddenly become anemic many are found dead. Even chronic phase, condition accompanied by mild but persistent parasitic burden characterized weight loss, widespread malaise and, some individuals, anemia. The objective this study was identify anthelmintic activity Croton tiglium seeds extract against comparison with drugs used internal external parasites. Methods: A performed evaluate methanolic using an adult worm motility test. Six graduated concentrations (25, 50, 75, 100, 125 150 mg/ml), were tested at different periods changes over time viability worms registered for 2, 4 6 hr. Normal RPMI-1640 medium ivermectin 0.1% as negative positive control, respectively. Also, histological conducted on integumentary occur it observed. Result: At 2 h post treatment, C. (100, 125, mg/ml) have caused significantly higher mortality compared other concentrations, while, similar nematodes within h. lower concentration (25 more lethal than control (RPMI-1640 medium) exposure. Each damages cuticle muscles contortus. increase proportional damage integumentary. present indicated all produced activity.